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Gut microbiome and its changes related to therapy of chronic diseases
Hurych, Jakub ; Cinek, Ondřej (advisor) ; Kolář, Milan (referee) ; Falt, Přemysl (referee)
This work examines the response of the gut microbiome to therapeutic interventions in three longitudinal studies of chronic gastrointestinal diseases: Crohn's disease, celiac autoimmunity and irritable bowel syndrome. Multiple methods of stool microbiome analysis (especially massively parallel 16S rDNA or 18S rDNA sequencing and metagenomic sequencing) followed by bioinformatic and statistical analysis were used. In Crohn's disease, we detected a previously undescribed secondary nature of changes in the gut bacteriome after anti-TNF treatment. In celiac disease autoimmunity, where previous works described an effect of probiotic intervention on serological markers of the disease, the gut bacteriome and metabolome, we described the absence of significant changes in beneficial gut protozoa. In irritable bowel syndrome, we observed a significant response of the bacteriome after administering four doses of mixed microbiota transplantation but no response in the reduction of clinical symptoms. The results of these studies could contribute to a better understanding of the gut microbiome's role in the pathogenesis of these serious diseases. Keywords: microbiome, Crohn's disease, celiac disease, irritable bowel syndrome
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Monitoring of cereal products uptake in a sample of Czech population
Vlachová, Martina ; Hurtová, Jana (referee) ; Márová, Ivana (advisor)
This bachelor’s thesis was focused on monitoring of consumption of cereal products and analysis of some of their active components. In theoretical part an overwiev of cereals and their composition was introduced. In experimental part a set of analytical parameters was obtained in selected cereal products: total phenolics, total flavonoids and antioxidant activity. The highest level of these active substances was detected in products containing chocolate and forest fruit. Further, 161 completed consumer questionnaires were collected. From these data common knowledge in consumers, their shopping conventions and individual products preferences were evaluated.
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Methods of assesment and treatment of coeliac disease
Valkus, Martin ; Omelková, Jiřina (referee) ; Vránová, Dana (advisor)
This diploma thesis deals with methods of determining and treatment of coeliac disease. In the theoretical part of the work sums up informations about coeliac disease - history, prevalence, etiopathogenesis, immunopathogenesis, possibilities of diagnosis, discusses about gluten-free diet, mentions legislation of the Czech Republic and the European Union and compares expensiveness of gluten-free diet and normal diet. The experimental part of this thesis compares PCR, ELISA and indirect immunofluorescence methods for assesment of determination of genetic predisposition and antibodies in coeliac disease (greatest emphasis was placed on the comparison of antibodies against gliadin and deamidated gliadin antibodies in IgA and IgG).
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Speciální druhy pečiva a možnosti inovace receptur
Janečková, Šárka
Thesis is focused on the production of baked goods for population groups suffering from diseases that require special dietary therapy. Subject of the literature research was to get acquainted with three of the most serious of them - celiac disease, Crohn's disease and diabetes and a consequent summary of the individual dietary requirements related to the diseases. The aim was to create an overview of basic, innovative and non-traditional ingredients suitable for the production of special baked goods for people suffering from these diseases. Practical part of the work was based on the development of six recipes for the production of bakery products using non-traditional ingredients, followed by successful execution of baking expe-riment, instrumental analysis of samples and sensory evaluation of the products. The baking experiment resulted in 3 groups of baked goods - gluten-free, non-residual and low-carbohydrate. The best volume, firmness and sensory properties were achieved in the gluten-free bakery products, which were very similar to the conventional bakery products and were also judged to be the most sensory ac-ceptable based on the sensory questionnaires. For the other two groups of bakery products, the results of the measurements and sensory evaluation were not very sa-tisfactory, although very interesting crust and crumb colours were achieved in the-se samples due to the used ingeridents.
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Determination of selected elements in cereal flours and their gluten-free alternatives
Venclová, Veronika ; Hraníček, Jakub (advisor) ; Nováková, Eliška (referee)
This study deals with the determination of calcium, magnesium, phosphorus, potassium, sodium, iron, zinc, copper, manganese and molybdenum in cereal gluten flours and its gluten-free alternatives. The flour samples were digested using a microwave device and the elements in solution were subsequently determined using an inductively coupled plasma mass spectrometer. Optimization of the digestion of the selected flours was performer prior to the actual determination of the elements. The aim of the work was to determine the above listed elements in the flour samples and to establish a basic overview of the elemental composition of the different flours. This overview and comparison of the different flours is intended to help the people with celiac disease to choose gluten-free alternatives to wheat flour. Keywords Inductively coupled plasma mass spectrometry, microwave digestion, calcium, magnesium, phosphorus, potassium, sodium, iron, zinc, copper, manganese, molybdenum, wheat flour, gluten-free flours, celiac disease
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Mechanisms of antigen presentation in the etiopathogenesis of celiac disease
Hudec, Michael ; Černá, Marie (advisor) ; Hrdý, Jiří (referee) ; Slavčev, Antonij (referee)
1 ABSTRACT Celiac disease (CeD) is a chronic autoimmune disease that develops as a response of the immune system to the presence of gluten in the small intestine. CeD is manifested not only by classic intestinal symptoms: abdominal pain, constipation or diarrhea, as well as complex less common symptoms: anemia, osteoporosis, psychiatric disorders or menstrual cycle disorders. HLA risk alleles predisposing to origin of celiac disease are HLA-DQ2 (DQA1*05:01 / DQB1*02:01) and HLA-DQ8 (DQA1*03:01 / DQB1*03:02). There are other celiac disease-associated polymorphisms outside of HLA locus (6p21.3) that are located in 5q32 and 19p13 regions with unclear connection to CeD development. HLA class II glycoproteins are expressed on antigen presenting cells (APC) that include dendritic cells, macrophages and B cells. Monocytes are one of several possible dendritic cell precursors that circulate in the bloodstream. Deviations in the frequency of intermediate monocytes are directly associated with autoimmune disorders such as Crohn's disease or rheumatoid arthritis. It is known that the monocytes of CeD patients show pro-inflammatory reaction in the presence of gluten. It means that, in the context of CeD, the response to gluten arises earlier than the activation of gluten-specific T cells. The conventional way of direct...
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How social networks affect celiacs'diets
Pretlová, Anna ; Háša, Marek (advisor) ; Hájková, Karolína (referee)
This paper examines the influence of social networks on the diet of celiacs. The aim was to investigate how social networks, specifically Facebook, Instagram, YouTube, Twitter and Pinterest, can influence the preparation, purchase and consumption of gluten-free products. The chosen research method was quantitative analysis. Results were obtained from Czech and a few Slovak celiacs and people with gluten allergy with help of a questionnaire survey followed by descriptive analysis. The questionnaire itself was disseminated on the social networks Instagram, Facebook and WhatsApp. The final sample included 264 from the original 279 respondents. The results show that the use of social networking sites is popular among patients with celiac disease and gluten-free diets and that users benefit from it. It was also revealed that the most useful social networks are Instagram amd Facebook. On the other hand, YouTube, Twitter and Pinterest fell completely in the evaluaion. The main reason for this is the small amount of beneficial information. When it comes to eating, celiacs are mainly looking for new recipes, products and places that offer gluten-free meals, according to the results. In addition to extend dietary horizons, social networks have also provent to contain important information about the disease...
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Screening of probiotics bacterial strains aimed at proof of gluten degradation
Korvasová, Lucie ; Obruča, Stanislav (referee) ; Ryšávka, Petr (advisor)
No other treatment for celiac disease and other gluten-related disorders has been found so far except for a lifelong diet, which is significantly restrictive for the patient. Moreover, it is not always possible to adhere strictly to this diet, mainly due to random gluten ingestion, which leads to health complications associated with these diseases. Currently, vaccination is being tested as an alternative treatment, and there is also increasing attention paid to enzymes that could break down the immunogenic part of gluten, specifically in its gliadin component. This thesis examined bacteria that could potentially break down this sequence in gliadin and thereby reduce its concentration during detection. A considerable proportion of the tested bacteria belong to probiotics to ensure their future use for humans is safe. To obtain data, the fact was utilized that as bacteria grow, the turbidity increases proportionally, which was subsequently detected to determine the amount of bacterial growth. The RIDASCREEN® Gliadin competitive kit was used for the gliadin detection test, which works on the principle of a competitive ELISA method with R5 antibodies. The result of the study was the finding that only the bacteria Bacillus subtilis was able to break down gliadin among the examined bacteria. It was also measured how much gliadin the bacteria could break down at different concentrations, and according to the data, it was evaluated that it is appropriate to adapt the culture to the presence of gliadin for a greater amount of broken gliadin.
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Potraviny pro zvláštní výživu-potraviny pro osoby s nesnášenlivostí lepku
Klusová, Petra
The aim of this bachelor thesis is to provide an overview of the topic Food for special nutrition, food for people with gluten intolerance. In the thesis, I focus on the labeling of gluten-free food and its nutritional value in comparison with food containing gluten. Among the gluten-free food we count those which contain more than 10 mg of gliadin per 100 g dry weight. The composition and labeling of gluten-free food is regulated by Commission Regulation (EC) 41/2009. Further, I focus on the origin of celiac disease, its development, symptoms and treatment. Celiac disease is caused by toxic effect of gluten particles, alpha gliadin. The body forms antibodies that affect enterocytes that are present in the lining of the small intestine.
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Awareness of general nurses about the treatment of celiak disease
HOLEJŠOVSKÁ, Petra
Abstract Objective: The bachelor's thesis deals with the awareness of general nurses about the treatment of celiac disease. The first chapter of the theoretical part is focused on the digestive system, small and large intestine. The following chapter is devoted to the definition of celiac disease, its history and clarification of the concept of gluten. The next chapter presents the symptoms, diagnosis and treatment of celiac disease and the health risks associated with it. The fourth chapter is devoted to gluten-free foods, their labeling, basic rules for buying gluten-free foods and preparing gluten-free food. The fifth chapter is focused on the contributions of health insurance companies to gluten-free food. The following chapter deals with the hospitalization of a celiac in a medical facility and provides some tips for hospitalization. The last chapter of the theoretical part deals with education, the role of the general nurse in the education of patients with celiac disease, forms of education and lists the most common myths associated with celiac disease. In the empirical part, the first goal was to map the awareness and knowledge of general nurses about celiac disease, its symptoms, manifestations and treatment. The second goal was to map the specifics of nursing care for patients with celiac disease. Method of achieving the goal: The set goals were achieved through a research survey, which was carried out using a qualitative approach, the method of questioning. The method of semi-structured interview was chosen for data collection. The examined group consisted of eight general nurses working in inpatient departments at the Tábor Hospital, a.s. Scientific benefits of the work: The research shows that general nurses have awareness and knowledge of celiac disease, its symptoms, manifestations and treatment, although the answers to some questions were only partial, sometimes inaccurate and in minimal cases incorrect. The identified specifics of nursing care for celiacs can be used in hospital facilities to improve the quality of care provided. Furthermore, the work can be used by people who are interested in obtaining information related to celiac disease and a gluten-free diet. Acquired knowledge and conclusions: The research survey revealed the specifics of nursing care for people with celiac disease, which are mainly associated with the gluten-free diet provided. Furthermore, celiac disease has been found to be incorrectly associated with allergies.
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